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Anti-MAP2K6 PHOSPHO Antibody
Also for MAP2K6 (NM_002758)
|The antiserum was produced against synthesized phosphopeptide derived from humanMKK6 around the phosphorylation site of serine 207 (V-D-SP-V-A).|
||WB: 1:500~1:1000, IHC: 1:50~1:100
|phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with UV, using MKK antibody (Lanes 1 and 2) and MKK (phospho-Ser207) antibody (Lanes 3 and 4).
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MKK6 (phospho-Ser207) antibody.