The antiserum was produced against synthesized phosphopeptide derived from humanGATA1 around the phosphorylation site of serine 310 (K-A-SP-G-K).
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clone Name
Isotype
Species Reactivity
Human, Mouse, Rat
Concentration
1ug/ul
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:500~1:1000
Background
Related Pathway
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Western blot analysis of extract from COS7 cells untreated or treated with EPO using GATA1 antibody (Lanes 1 and 2) and GATA1 (phospho-Ser310) antibody (Lanes 3 and 4).
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