Western blot analysis of CTHRC1 protein expression in cultured melanocytes and melanoma cell lines. CTHR1 protein is readily detectable from three melanoma cell lines (KZ-28, MMRU and PMWK) but not detectable from any normal melanocytes (HEMC1-C3).
Immunohistochemistry analysis of melanoma tissues by anti-CTHRC1 antibody. CTHR1 protein is negative in melanoma in situ (MIS), positive in primary melanoma (PM), and very strong positive in metastatic melanoma (MM).
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