Home Antibody All anti-TP53BP1 antibodies
Also for TP53BP1 (NM_005657)
|The epitope recognized by this antibody maps to a region between residues 350 and 400 of human 53BP1 [NP_005648.1 (GeneID 7158)].|
|WB, IF, FC
||WB, FC, ICC, IF, IP
|Tris-citrate/phosphate buffer, pH 7 to 8 and 0.09% Sodium Azide|
|Immunogen affinity purified
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Western Blot: 53BP1 Antibody - Detection of Human [deleted mouse] 53BP1 by Western Blot Sample: Whole cell lysate (20 ug/lane) from U2OS or 293T cells resolved on a 3 to 8% tris-acetate gel. Anti-53BP1 used at 0.5 ug/ml.
Immunofluorescence: 53BP1 antibody - 53BP1 foci in proliferating MO59K cells; both normal and exposed to 10 Gy of IR and double-color immunofluorescence stained after 2 hours. Images captured in a Kodak digital image system on a Leica fluorescent microscope.
immunofluorescence: 53BP1 amtibody - 53BP1 foci in proliferating MEFs; both normal and exposed to 10 Gy of IR and double-color immunofluorescence stained after 2 hours. Images captured in a Kodak digital image system on a Leica fluorescent microscope.
Flow Cytometry: 53BP1 Antibody - 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 ug/ml anti-53BP1 in a 150 ul reaction. Isotype control (black), anti-53BP1 (red).