Home All anti-IL1B antibodies
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Also for IL1B (NM_000576)
|Recombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 15kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).|
|Canine, Human, Primate
||WB, FC, IHC, ELISA
|0.02M Potassium Phosphate, 0.15M sodium chloride, pH 7.2 and |
|IgG purified (Protein A or G Sepharose)
|Homo sapiens interleukin 1, beta (IL1B)|
|IL-1; IL1-BETA; IL1F2|
Cytokine-cytokine receptor interaction
Hematopoietic cell lineage
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western Blot: IL1 beta Antibody - Immunoblotting. Anti-Human IL-1B was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3,000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.