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Anti-TH PHOSPHO Antibody
|Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 conjugated to KLH|
|human, mouse, rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1,000, IHC: 1:1000, IF: 1:1,000
|100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
|Rattus norvegicus tyrosine hydroxylase (Th)|
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Western blot of recombinant phospho- and dephospho-TH showing selective immunolabeling by the phospho-specific antibody of the ~60k TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and dephospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.
Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and phospho-specific tyrosine hydroxylase (phospho-TH) antibodies. The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.