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Anti-RAF1 PHOSPHO Antibody
Also for RAF1 (NM_012639)
|Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser301 conjugated to KLH|
||0.5~1.0 mg/ml (Lot Dependent)
|100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
|Rattus norvegicus v-raf-leukemia viral oncogene 1 (Raf1)|
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Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf-1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: Î»-Ptase). The blot is identical to the control except that it was incubated in Î»-Ptase (1200 units for 30 min) before being exposed to the phospho Ser301 Raf-1 antibody. The immunolabeling of Raf-1 is completely eliminated by treatment with Î»-Ptase.