Home Antibody All anti-IKBKB antibodies
Also for IKBKB (NM_001556)
|Recombinant fragment corresponding to a region within amino acids 493 and 739 of IKK beta (Uniprot ID#O14920)|
|Human (Predicted: Rhesus Monkey)
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:10000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Rhesus Monkey (100%)
|Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (IKBKB), transcript variant 1|
|IKK-beta; IKK2; IKKB; IMD15; NFKBIKB|
Entrez Gene 3551 Human
Entrez Gene 704683 Monkey
|NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]|
|Protein KinaseTranscription FactorsDruggable Genome MAPK signaling pathwayChemokine signaling pathwayApoptosisToll-like receptor signaling pathwayNOD-like receptor signaling pathwayRIG-I-like receptor signaling pathwayMore Pathways >> |
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IKK beta antibody detects IKBKB protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. IKK beta antibody (TA308825) dilution: 1:1000
Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. TA308825 diluted at 1:1000.
IKK beta antibody detects IKK beta protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of DDDDK-human IKBKB-transfected 293T cells. 7.5 % SDS-PAGE. IKK beta antibody (TA308825) dilution: 1:5000
Immunohistochemical analysis of paraffin-embedded Colon ca, using IKK beta(TA308825) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using IKK beta(TA308825) antibody at 1:200 dilution.