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Home Antibody All anti-IKBKB antibodies

Anti-IKBKB Antibody

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SKU Description Amount Price Availability*  
TA308825 Rabbit polyclonal antibody to IKK beta (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta) 100ul $325 3-7 Days
LC419865 IKBKB HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(3)
IHC(1)
IF(1)
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Also for IKBKB (NM_001556)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 493 and 739 of IKK beta (Uniprot ID#O14920)
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Rhesus Monkey) Concentration0.41mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:10000
Predicted MW Explanation 87kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Rhesus Monkey (100%)

Reference Data

Target NameHomo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (IKBKB), transcript variant 1
Alternative NameIKK-beta; IKK2; IKKB; IMD15; NFKBIKB
Database LinkNP_001547
Entrez Gene 3551 Human
Entrez Gene 704683 Monkey
FunctionNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
Related PathwayProtein KinaseTranscription FactorsDruggable Genome MAPK signaling pathwayChemokine signaling pathwayApoptosisToll-like receptor signaling pathwayNOD-like receptor signaling pathwayRIG-I-like receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

IKK beta antibody detects IKBKB protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. IKK beta antibody (TA308825) dilution: 1:1000
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Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. TA308825 diluted at 1:1000.
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IKK beta antibody detects IKK beta protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of DDDDK-human IKBKB-transfected 293T cells. 7.5 % SDS-PAGE. IKK beta antibody (TA308825) dilution: 1:5000
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Immunohistochemical analysis of paraffin-embedded Colon ca, using IKK beta(TA308825) antibody at 1:500 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed A431, using IKK beta(TA308825) antibody at 1:200 dilution.
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