Home Antibody All anti-ACHE antibodies
Also for ACHE (NM_000665)
|Synthetic peptide corresponding to a region within amino acids 551 and 614 of AChE (Uniprot ID#P22303)|
|Human (Predicted: Rat, Cat, Guinea pig, Xenopus laevis, Zebrafish, Bovine, Rhesus Monkey)
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Rat (93%), Zebrafish (86%), Xenopus laevis (85%), Cat (100%), Rhesus Monkey (100%), Bovine (100%), Guinea pig (100%)
|Homo sapiens acetylcholinesterase (Yt blood group) (ACHE), transcript variant E4-E6|
|ACEE; ARACHE; N-ACHE; YT|
Entrez Gene 43 Human
Entrez Gene 83817 Rat
Entrez Gene 713370 Monkey
|Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq]|
|Druggable Genome Glycerophospholipid metabolism|
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Sample (30 ug of whole cell lysate). A: Hep G2 . B: Molt-4 . 7.5% SDS PAGE. TA308775 diluted at 1:500
AChE antibody [C3], C-term detects AChE protein at cytoplasm dot-like staining on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. AChE antibody [C3], C-term (TA308775) dilution: 1:250.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AChE(TA308775) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.