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Anti-OGT Antibody

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SKU Description Amount Price Availability*  
TA308773 Rabbit Polyclonal antibody to O-GlcNAc transferase (O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)) 100ul $325 3-7 Days
LC405662 OGT HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(2)

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 1 and 280 of O-GlcNAc transferase (Uniprot ID#O15294)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Rat, Xenopus laevis, Xenopus Tropicalis, Pig, Bovine) Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsIHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 116kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Rat (100%), Xenopus laevis (100%), Xenopus Tropicalis (100%), Pig (100%), Bovine (100%)

Reference Data

Target NameHomo sapiens O-linked N-acetylglucosamine (GlcNAc) transferase (OGT), transcript variant 2
Alternative NameHRNT1; O-GLCNAC
Database LinkNP_858059
Entrez Gene 8473 Human
Entrez Gene 108155 Mouse
Entrez Gene 26295 Rat
FunctionO-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
Related PathwayDruggable Genome O-Glycan biosynthesisMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. TA308773 diluted at 1:1000.
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Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. TA308773 diluted at 1:1000
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O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. O-GlcNAc transferase antibody [N1N2], N-term (TA308773) dilution:
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O-GlcNAc transferase antibody [N1N2], N-term detects OGT protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. O-GlcNAc transferase antibody [N1N2], N-term (TA308773) dilution: 1:500.
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