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Home Antibody All anti-PRKAA2 antibodies

Anti-PRKAA2 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308756
  • Rabbit Polyclonal antibody to AMPK alpha 2 (protein kinase, AMP-activated, alpha 2 catalytic subunit)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416772) , 20ug Explanation
100ul 325 3-7 Days
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WB(2)
IP(1)
IHC(1)
IF(1)
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Also for PRKAA2 (NM_006252)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2 (Uniprot ID#P54646)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Chicken, Pig, Rat, Bovine, Xenopus tropicalis) Concentration0.55mg/ml
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 62kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Chicken (96%), Pig (99%), Rat (98%), Bovine (99%), Xenopus tropicalis (96%)

Reference Data

Target NameHomo sapiens protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2)
Alternative NameAMPK; AMPK2; AMPKa2; PRKAA
Database LinkNP_006243
Entrez Gene 5563 Human
Entrez Gene 108079 Mouse
Entrez Gene 78975 Rat
FunctionThe protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq]
Related PathwayProtein KinaseDruggable Genome Regulation of autophagymTOR signaling pathwayInsulin signaling pathwayAdipocytokine signaling pathwayHypertrophic cardiomyopathy (HCM)

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Sample (30 ug of whole cell lysate). . A:NIH-3T3. 7.5% SDS PAGE. . TA308756 diluted at 1:1000.
WB Image
AMPK alpha 2 antibody detects PRKAA2 protein by Western blot analysis. A. 30 ug A431 whole cell lysate/extract. B. 30 ug HeLa whole cell lysate/extract. C. 30 ug A375 whole cell lysate/extract. 7.5 % SDS-PAGE. AMPK alpha 2 antibody (TA308756) dilution: 1:
IHC Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using AMPK alpha 2(TA308756) antibody at 1:500 dilution.
IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using AMPK alpha 2(TA308756) antibody at 1:200 dilution.
IP Image
AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha

 

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