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Also for PRKAA2 (NM_006252)
|Recombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2 (Uniprot ID#P54646)|
|Human, Mouse (Predicted: Chicken, Pig, Rat, Bovine, Xenopus tropicalis)
|WB, IHC, IF, IP
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Chicken (96%), Pig (99%), Rat (98%), Bovine (99%), Xenopus tropicalis (96%)
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Sample (30 ug of whole cell lysate). . A:NIH-3T3. 7.5% SDS PAGE. . TA308756 diluted at 1:1000.
AMPK alpha 2 antibody detects PRKAA2 protein by Western blot analysis. A. 30 ug A431 whole cell lysate/extract. B. 30 ug HeLa whole cell lysate/extract. C. 30 ug A375 whole cell lysate/extract. 7.5 % SDS-PAGE. AMPK alpha 2 antibody (TA308756) dilution: 1:
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using AMPK alpha 2(TA308756) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using AMPK alpha 2(TA308756) antibody at 1:200 dilution.
AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha