Home All anti-PDHA1 antibodies
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Also for PDHA1 (NM_000284)
|Recombinant protein fragment contain a sequence corresponding to a region within amino acids 39 and 295 of Pyruvate Dehydrogenase E1 alpha|
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography. (Protein A or G Sepharose)
|Homo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein, transcript variant 1|
|PDHA; PDHCE1A; PHE1A|
|The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 22.214.171.124); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 126.96.36.199). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]|
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Sample(30 ug of whole cell lysate) A:HepG2 B:MOLT4 (anti-PDHA1 antibody diluted at 1:1000)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pyruvate Dehydrogenase E1 alpha(anti-PDHA1 antibody) antibody at 1:200 dilution.