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Home Antibody All anti-PFKM antibodies

Anti-PFKM Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308655
  • Rabbit Polyclonal antibody to PFK (muscle) (phosphofructokinase, muscle)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424822) , 20ug Explanation
100ul 325 3-7 Days
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WB(2)
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Also for PFKM (NM_000289)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 1 and 253 of PFK (muscle) (Uniprot ID#P08237)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Chicken, Dog, Pig, Rat, Xenopus laevis, Bovine) Concentration0.92mg/ml
Guaranteed Application *WB Suggested DilutionsWB:1:1000-1:10000
Predicted MW Explanation 85kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Chicken (88%), Dog (97%), Pig (95%), Rat (94%), Xenopus laevis (89%), Bovine (96%)

Reference Data

Target NameHomo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4
Alternative NameATP-PFK; GSD7; PFK-1; PFK1; PFKA; PFKX; PPP1R122
Database LinkNP_000280
Entrez Gene 5213 Human
Entrez Gene 18642 Mouse
Entrez Gene 65152 Rat
Entrez Gene 403849 Dog
FunctionThe PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]
Related PathwayDruggable Genome Glycolysis / GluconeogenesisPentose phosphate pathwayFructose and mannose metabolismGalactose metabolismMetabolic pathways

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WB Image
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308655 diluted at 1:2000.
WB Image
Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. TA308655 diluted at 1:5000.

 

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