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Home Antibody All anti-NKX2-5 antibodies

Anti-NKX2-5 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA308654 Rabbit Polyclonal antibody to Nkx2.5 (NK2 transcription factor related, locus 5 (Drosophila)) 100ul $325 3-7 Days
LC401397 NKX2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for NKX2-5 (NM_004387)
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OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 1 and 218 of Nkx2.5 (Uniprot ID#P52952)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse Concentration1mg/ml
Guaranteed Application *WB, IF Suggested DilutionsICC/IF:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 35kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.

Reference Data

Target NameHomo sapiens NK2 homeobox 5 (NKX2-5), transcript variant 1
Alternative NameCHNG5; CSX; CSX1; HLHS2; NKX2.5; NKX2E; NKX4-1; VSD3
Database LinkNP_004378
Entrez Gene 1482 Human
Entrez Gene 18091 Mouse
FunctionHomeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Sample(30 ug whole cell lysate). A:H1299. B:Raji . 10% SDS PAGE. TA308654 diluted at 1:1000
Sample (50 ug of whole cell lysate). A: mouse heart. 10% SDS PAGE. TA308654 diluted at 1:1000.
Nkx2.5 antibody detects NKX2-5 protein by Western blot analysis. A. 50 ug rat heart lysate/extract. 10 % SDS-PAGE. Nkx2.5 antibody (TA308654) dilution: 1:500
Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Nkx2.5(TA308654) antibody at 1:200 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NKX2-5(TA308654) antibody at 1:200 dilution.


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