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Home Antibody All anti-PSME3 antibodies

Anti-PSME3 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308562
  • Rabbit Polyclonal antibody to PSME3 (proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki))
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417076) , 20ug Explanation
100ul 325 3-7 Days
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WB(3)
IHC(1)
IF(1)
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Also for PSME3 (NM_005789)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant fragment contain a sequence corresponding to a region within amino acids 1 and 254 of PSME3
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Pig) Concentration0.65mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 30kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Pig (100%)

Reference Data

Target NameHomo sapiens proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) (PSME3), transcript variant 1
Alternative NameHEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; REG-GAMMA
Database LinkNP_005780
Entrez Gene 10197 Human
Entrez Gene 19192 Mouse
FunctionThe 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq]
Related PathwayStem cell - Pluripotency ProteasomeAntigen processing and presentation

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WB Image
Sample (30 ug of whole cell lysate). A: A549. B: H1299. C: HCT116. D: MCF-7. 12% SDS PAGE. TA308562 diluted at 1:2000.
WB Image
Sample (30 ug of whole cell lysate). A: NIH-3T3. B: JC. C: BCL-1. 12% SDS PAGE. TA308562 diluted at 1:2000.
WB Image
Sample (30 ug of whole cell lysate). A: PC-12. 12% SDS PAGE. TA308562 diluted at 1:2000.
IHC Image
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using PSME3(TA308562) antibody at 1:100 dilution.
IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSME3(TA308562) antibody at 1:500 dilution.

 

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