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Home Antibody All anti-RFC4 antibodies

Anti-RFC4 Antibody

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SKU Description Amount Price Availability*  
TA308528 Rabbit Polyclonal antibody to RFC4 (replication factor C (activator 1) 4, 37kDa) 100ul $325 3-7 Days
LC419019 RFC4 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(1)
IF(1)

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 18 and 323 of RFC4 (Uniprot ID#P35249)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Rat, Xenopus laevis, Xenopus tropicalis) Concentration0.62mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 40kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Rat (92%), Xenopus laevis (84%), Xenopus tropicalis (87%)

Reference Data

Target NameHomo sapiens replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1
Alternative NameA1; RFC37
Database LinkNP_002907
Entrez Gene 5984 Human
Entrez Gene 106344 Mouse
Entrez Gene 288003 Rat
FunctionThe elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationNucleotide excision repairMismatch repair

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Sample (30 ug of whole cell lysate). A: H1299. B: Hela. C: Hep G2 . 10% SDS PAGE. TA308528 diluted at 1:1000.
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Sample (50 ug of whole cell lysate). A: mouse liver. 10% SDS PAGE. TA308528 diluted at 1:500.
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Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using RFC4(TA308528) antibody at 1:100 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4(TA308528) antibody at 1:200 dilution.
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