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Home Antibody All anti-ACTN4 antibodies

Anti-ACTN4 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308476
  • Rabbit Polyclonal antibody to alpha Actinin 4 (actinin, alpha 4)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417652) , 20ug Explanation
100ul 325 3-7 Days
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WB(4)
IHC(1)
IF(1)
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Also for ACTN4 (NM_004924)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 206 and 542 of alpha Actinin 4 (Uniprot ID#O43707)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Rhesus Monkey, Bovine) Concentration1.03mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 105kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Rhesus Monkey (100%), Bovine (100%)

Reference Data

Target NameHomo sapiens actinin, alpha 4 (ACTN4)
Alternative NameACTININ-4; FSGS; FSGS1
Database LinkNP_004915
Entrez Gene 81 Human
Entrez Gene 60595 Mouse
Entrez Gene 693666 Monkey
Function Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]
Related PathwayDruggable Genome Focal adhesionAdherens junctionTight junctionLeukocyte transendothelial migrationRegulation of actin cytoskeletonSystemic lupus erythematosusMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308476 diluted at 1:1000.
WB Image
alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5% SDS-PAGE. alpha Actinin 4 antibody [N2C1], Internal (TA308476) dilution: 1:3000.
WB Image
Sample (30 ug of whole cell lysate). A: A431 . B: Hep G2 . 7.5% SDS PAGE. TA308476 diluted at 1:1000.
WB Image
Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. TA308476 diluted at 1:1000.
IHC Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(TA308476) antibody at 1:500 dilution.
IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4(TA308476) antibody at 1:200 dilution.

 

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