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Anti-TPI1 Antibody


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SKU Description Amount Price Availability*  
TA308459 Rabbit Polyclonal antibody to Triosephosphate isomerase (triosephosphate isomerase 1) 100ul $325 3-7 Days
LC424764 TPI1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenSynthetic peptide corresponding to a region within amino acids 187 and 249 of Triosephosphate isomerase (Uniprot ID#P60174)
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Yeast, Xenopus Tropicalis, Dog, Chicken, Chimpanzee, Bovine) Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsELISA:; ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 27kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Yeast (83%), Xenopus Tropicalis (92%), Dog (100%), Chicken (92%), Chimpanzee (100%), Bovine (100%)

Reference Data

Target NameHomo sapiens triosephosphate isomerase 1 (TPI1), transcript variant 1
Alternative NameHEL-S-49; TIM; TPI; TPID
Database LinkNP_000356
Entrez Gene 7167 Human
Entrez Gene 477711 Dog
FunctionThis gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Related Pathway Glycolysis / GluconeogenesisFructose and mannose metabolismInositol phosphate metabolismMetabolic pathways

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WB Image
Triosephosphate isomerase antibody [C2C3], C-term detects TPI1 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell ly

WB Image
Triosephosphate isomerase antibody [C2C3], C-term detects TPI1 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. Triosephosphate isomerase antibody [C2C3], C-term (TA308459)

WB Image
Sample(30 ug of whole cell lysate). A:A431. B:H1299. 12% SDS PAGE. TA308459 diluted at 1:2000

IHC Image
Immunohistochemical analysis of paraffin-embedded SNU-16 xenograft, using TPI1 (TA308459) antibody at 1:100 dilution.

IF Image
Triosephosphate isomerase antibody [C2C3], C-term detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase protein stained


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