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Anti-OGT Antibody

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SKU Description Amount Price Availability*  
TA308359 Rabbit Polyclonal antibody to O-GlcNAc transferase (O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)) 100ul $325 In Stock
LC430555 OGT HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(3)
IHC(1)
IF(1)

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 223 and 502 of O-GlcNAc transferase (Uniprot ID#O15294)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Pig, Bovine) Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 116kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Pig (100%), Bovine (100%)

Reference Data

Target NameHomo sapiens O-linked N-acetylglucosamine (GlcNAc) transferase (OGT), transcript variant 2
Alternative NameHRNT1; O-GLCNAC
Database LinkNP_858059
Entrez Gene 8473 Human
Entrez Gene 108155 Mouse
FunctionO-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
Related PathwayDruggable Genome O-Glycan biosynthesisMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug A431 whole cell extract. C. 30 ug HeLa whole cell extract. D. 30 ug HepG2 whole cell extract. 7.5 % SDS-PAGE. O-GlcNAc
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O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 50 ug mouse brain extract. 5 % SDS-PAGE. O-GlcNAc transferase antibody (TA308359) dilution: 1:1000
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O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 30 ug PC-12 whole cell extract. 5 % SDS-PAGE. O-GlcNAc transferase antibody (TA308359) dilution: 1:1000
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Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using O-GlcNAc transferase (TA308359) antibody at 1:100 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using O-GlcNAc transferase(TA308359) antibody at 1:200 dilution.
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