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Home Antibody All anti-PNPT1 antibodies

Anti-PNPT1 Antibody

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SKU Description Amount Price Availability*  
TA308058 Rabbit Polyclonal antibody to PNPase (polyribonucleotide nucleotidyltransferase 1) 100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 1 and 783 of PNPase (Uniprot ID#Q8TCS8)
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Xenopus tropicalis) Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 86kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Xenopus tropicalis (80%)

Reference Data

Target NameHomo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1)
Alternative NameCOXPD13; DFNB70; old-35; OLD35; PNPASE
Database LinkNP_149100
Entrez Gene 87178 Human
FunctionPNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM]
Related Pathway Purine metabolismPyrimidine metabolismRNA degradation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. TA308058 diluted at 1:1000.
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PNPase antibody detects PNPase protein at mitochondria on human normal colon by immunohistochemical analysis. Sample: Paraffin-embedded human normal colon. PNPase antibody (TA308058) dilution: 1:250.
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Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PNPase(TA308058) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2500.
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