Home Antibody All anti-FH antibodies
|Recombinant fragment corresponding to a region within amino acids 195 and 473 of fumarate hydratase (Uniprot ID#P07954)|
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Homo sapiens fumarate hydratase (FH)|
|FMRD; HLRCC; LRCC; MCL; MCUL1|
Entrez Gene 2271 Human
|The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]|
|Druggable Genome Citrate cycle (TCA cycle)Metabolic pathwaysPathways in cancerRenal cell carcinoma|
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Fumarate hydratase antibody detects FH protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 7.5% SDS-PAGE. Fumarate hydratase antibody (TA307996) dilution: 1:1000.
Fumarate hydratase antibody detects Fumarate hydratase protein by Western blot analysis. A.50 ug rat liver lysate/extract. 10 % SDS-PAGE. Fumarate hydratase antibody (TA307996) dilution: 1:5000
Sample (30 ug of whole cell lysate). A: HeLa. B: Hep G2 . 10% SDS PAGE. TA307996 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase (TA307996) antibody at 1:500 dilution.
Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37Ã¢ for 30 min. Green: Fumarate hydratase protein stained by Fumarate hyd