Home Antibody All anti-ATP6V1H antibodies
Also for ATP6V1H (NM_213620)
|Recombinant protein fragment contain a sequence corresponding to a region within amino acids 169 and 414 of human ATP6V1H|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB:1:500-1:3000; IHC:1:500; IF:1:100-1:500;
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography. (Protein A or G Sepharose)
|Homo sapiens ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 3|
|CGI-11; MSTP042; NBP1; SFD; SFDalpha; SFDbeta; VMA13|
|This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit. [provided by RefSeq]|
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Sample (30 ug of whole cell lysate) A: Hela (anti-ATP6V1H antibody diluted at 1:1000)
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ATP6V1H(anti-ATP6V1H antibody) antibody at 1:500 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using ATP6V1H(anti-ATP6V1H antibody) antibody at 1:200 dilution.