FH Rabbit Polyclonal Antibody

CAT#: TA307962

Rabbit Polyclonal antibody to Fumarate hydratase (fumarate hydratase)

Product Images

Fumarate hydratase antibody [N2C2], Internal detects FH protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 10% SDS-PAGE. Fumarate hydratase antibody [N2C2], Internal (TA307962) dilution: 1:1000.

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  Sample (30 ug of whole cell lysate). A: 293T. B: A431. 10% SDS PAGE. TA307962 diluted at 1:5000

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  Fumarate hydratase antibody [N2C2], Internal detects Fumarate hydratase protein by Western blot analysis. A.50 ug rat liver lysate/extract. 10 % SDS-PAGE. Fumarate hydratase antibody [N2C2], Internal (TA307962) dilution: 1:5000

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  Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using fumarate hydratase (TA307962) antibody at 1:500 dilution.

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  Immunofluorescence analysis of methanol-fixed HeLa, using Fumarate hydratase (TA307962) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 89 and 365 of Fumarate hydratase (Uniprot ID#P07954)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55 kDa
• Gene Name: fumarate hydratase
• Database Link:
  NP_000134
  Entrez Gene 2271 Human
  UniProt ID P07954
• Background: The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]
• Synonyms: FMRD; HLRCC; LRCC; MCL; MCUL1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Pathways in cancer, Renal cell carcinoma