Home Antibody All anti-RAG2 antibodies
Also for RAG2 (NM_000536)
|Recombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (Uniprot ID#P55895)|
|Human (Predicted: Mouse, Pig, Rabbit, Rat, Bovine)
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Mouse (85%), Pig (90%), Rabbit (91%), Rat (86%), Bovine (91%)
|Homo sapiens recombination activating gene 2 (RAG2), transcript variant 1|
Entrez Gene 5897 Human
Entrez Gene 19374 Mouse
Entrez Gene 295953 Rat
|This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq]|
|Druggable Genome Primary immunodeficiency|
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Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA307957 diluted at 1:1000.
RAG2 antibody detects RAG2 protein by Western blot analysis. A. 50 ug Rat thymus lysate/extract. 7.5 % SDS-PAGE. RAG2 antibody (TA307957) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using RAG2(TA307957) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2(TA307957) antibody at 1:200 dilution.