OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-RAG2 antibodies

Anti-RAG2 Antibody

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307957
  • Rabbit polyclonal antibody to RAG2 (recombination activating gene 2)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424659) , 20ug Explanation
100ul 325 3-7 Days
Add to Shopping Cart
WB(2)
IHC(1)
IF(1)

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (Uniprot ID#P55895)
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Mouse, Pig, Rabbit, Rat, Bovine) Concentration0.37mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Mouse (85%), Pig (90%), Rabbit (91%), Rat (86%), Bovine (91%)

Reference Data

Target NameHomo sapiens recombination activating gene 2 (RAG2), transcript variant 1
Alternative NameRAG-2
Database LinkNP_000527
Entrez Gene 5897 Human
Entrez Gene 19374 Mouse
Entrez Gene 295953 Rat
FunctionThis gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq]
Related PathwayDruggable Genome Primary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA307957 diluted at 1:1000.
WB Image
RAG2 antibody detects RAG2 protein by Western blot analysis. A. 50 ug Rat thymus lysate/extract. 7.5 % SDS-PAGE. RAG2 antibody (TA307957) dilution: 1:1000
IHC Image
Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using RAG2(TA307957) antibody at 1:100 dilution.
IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2(TA307957) antibody at 1:200 dilution.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title