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Anti-HUS1 Antibody EPR5132
Also for HUS1 (NM_004507)
|A synthetic peptide corresponding to residues in human HUS1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for ICC.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC.
|Homo sapiens HUS1 checkpoint homolog (S. pombe) (HUS1), transcript variant 1|
|HUS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. It forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and it is thought to be an early checkpoint signaling event.|
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Western blot - HUS1 antibody [EPR5132]; All lanes : Anti-HUS1 antibody [EPR5132] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : K562 cell lysate.Lane 3 : A431 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 32 kDa.Observed band size : 34 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HUS1 antibody [EPR5132]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab109371 at a dilution of 1/100.
Flow Cytometry - Anti-HUS1 antibody [EPR5132]; Overlay histogram showing HeLa cells stained with ab109371 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.