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Anti-TRIM28 Antibody EPR5217
Also for TRIM28 (NM_005762)
|A synthetic peptide corresponding to residues on the C-terminus in human TIF1-beta was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens tripartite motif containing 28 (TRIM28)|
|KAP1; RNF96; TF1B; TIF1B|
|TIF1-beta mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. It localizes to the nucleus and is thought to associate with specific chromatin regions. TIF1-beta is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.|
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Western blot - KAP1 antibody [EPR5217]; All lanes : Anti-KAP1 antibody [EPR5217] at 1/1000 dilution.Lane 1 : A431 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : PC-3 cell lysate.Lane 4 : F9 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 89 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAP1 antibody [EPR5217]; Immunohistochemical analysis of paraffin-embedded Human spleen tissue using ab109289 at a dilution of 1/250.
Immunocytochemistry/ Immunofluorescence - KAP1 antibody [EPR5217]; Immunofluorescent staining of HeLa cells using ab109289 at a dilution of 1/100.