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Home Antibody All anti-TRIM28 antibodies

Anti-TRIM28 Antibody EPR5217

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307866
  • Rabbit monoclonal antibody against TIF1-beta(clone EPR5217)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC417086) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for TRIM28 (NM_005762)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus in human TIF1-beta was used as an immunogen.
Clone NameEPR5217 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens tripartite motif containing 28 (TRIM28)
Alternative NameKAP1; RNF96; TF1B; TIF1B
Database LinkNP_005753
FunctionTIF1-beta mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. It localizes to the nucleus and is thought to associate with specific chromatin regions. TIF1-beta is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - KAP1 antibody [EPR5217]; All lanes : Anti-KAP1 antibody [EPR5217] at 1/1000 dilution.Lane 1 : A431 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : PC-3 cell lysate.Lane 4 : F9 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 89 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAP1 antibody [EPR5217]; Immunohistochemical analysis of paraffin-embedded Human spleen tissue using TA307866 at a dilution of 1/250.
IF Image
Immunocytochemistry/ Immunofluorescence - KAP1 antibody [EPR5217]; Immunofluorescent staining of HeLa cells using TA307866 at a dilution of 1/100.

 

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