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Home Antibody All anti-TRIM28 antibodies

Anti-TRIM28 Antibody EPR5216

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307862
  • Rabbit monoclonal antibody against TIF-1-beta(clone EPR5216)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417086) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(2)
IF(1)
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Also for TRIM28 (NM_005762)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human TIF-1-beta was used as an immunogen.
Clone NameEPR5216 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 89 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens tripartite motif containing 28 (TRIM28)
Alternative NameKAP1; PPP1R157; RNF96; TF1B; TIF1B
Database LinkNP_005753
Entrez Gene 10155 Human
Entrez Gene 21849 Mouse
Entrez Gene 116698 Rat
FunctionTIF-1-beta is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TIF-1-beta mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. This protein localizes to the nucleus and is thought to associate with specific chromatin regions (1).
Related PathwayProtein KinaseStem cell - PluripotencyTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - KAP1 antibody [EPR5216]; All lanes : Anti-KAP1 antibody [EPR5216] at 1/10000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : A431 cell lysates.Lane 3 : PC-3 cell lysates.Lane 4 : MCF7 cell lysates.Lysates/proteins at 10 ug per lane.Predicted band size : 89 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAP1 antibody [EPR5216]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using 1/250 TA307862.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAP1 antibody [EPR5216]; Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/250 TA307862.
IF Image
Immunocytochemistry/ Immunofluorescence - KAP1 antibody [EPR5216]; Immunofluorescent staining of HeLa cells using 1/100 TA307862.

 

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