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Anti-UPF1 Antibody EPR4681
Also for UPF1 (NM_002911)
|A synthetic peptide corresponding to residues in human RENT1 / hUPF1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1)|
|HUPF1; NORF1; pNORF1; RENT1; smg-2|
|RENT1 / hUPF1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. RENT1 / hUPF1 is located only in the cytoplasm. It interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for RENT1 / hUPF1.|
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Western blot - RENT1/hUPF1 antibody [EPR4681]; All lanes : Anti-RENT1/hUPF1 antibody [EPR4681] at 1/10000 dilution.Lane 1 : HuT-78 cell lysate.Lane 2 : Raji cell lysate.Lane 3 : SH-SY5Y cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Performed under reducing conditions.Predicted band size : 124 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RENT1/hUPF1 antibody [EPR4681]; ab109363, at 1/100, staining RENT1/hUPF1 in Human kidney tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RENT1/hUPF1 antibody [EPR4681]; ab109363, at 1/100, staining RENT1/hUPF1 in Human transitional cell carcinoma tissue by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - RENT1/hUPF1 antibody [EPR4681]; ab109363, at 1/100, staining RENT1/hUPF1 in HeLa cells by immunofluorescence.
Flow Cytometry-Anti-RENT1/hUPF1 antibody [EPR4681](ab109363); Overlay histogram showing HeLa cells stained with ab109363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.