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Anti-BNIP3 Antibody EPR4034
Also for BNIP3 (NM_004052)
|A synthetic peptide corresponding to residues in human NIP3 / BNIP3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens BCL2/adenovirus E1B 19kDa interacting protein 3 (BNIP3)|
|NIP3 / BNIP3 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 / BNIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.|
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Western blot - BNIP3 antibody [EPR4034]; All lanes : Anti-BNIP3 antibody [EPR4034] at 1/1000 dilution.Lane 1 : Jurkat cell lysate treated with etoposide.Lane 2 : Jurkat cell lysate.Lane 3 : SH-SY5Y cell lysate treated with camptothecin.Lane 4 : SH-SY5Y cell lysate.Lane 5 : MCF-7 cell lysate treated with Cocl2.Lysates/proteins at 10 µg per lane.Predicted band size : 22 kDa.Observed band size : 30 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BNIP3 antibody [EPR4034]; ab109362, at 1/50, staining BNIP3 in Human kidney tissue by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - BNIP3 antibody [EPR4034]; ab109362, at 1/100, staining BNIP3 in SH-SY5Y by immunofluorescence.