OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-ICAM-1 antibodies

Anti-ICAM-1 Antibody EPR4776

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307840
  • Rabbit monoclonal antibody against ICAM-1(clone EPR4776)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400073) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(2)
FC(1)
spacer
Also for ICAM-1 (NM_000201)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA recombinant protein corresponding to a fragment near the C-terminus of human ICAM-1 was used as an immunogen.
Clone NameEPR4776 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC.

Reference Data

Target NameHomo sapiens intercellular adhesion molecule 1 (ICAM1)
Alternative NameBB2; CD54; P3.58
Database LinkNP_000192
Entrez Gene 3383 Human
FunctionAntigen-specific cell contacts in the immune system are strengthened by antigen-nonspecific interactions, mediated in part by lymphocyte-function associated (LFA) antigens. ICAM-1 (intercellular adhesion molecule-1) has been defined as a ligand for LFA-1. Monoclonal antibodies to ICAM-1 block T lymphocyte adhesion to fibroblasts and endothelial cells; they disrupt the interaction between cytotoxic T cells and target cells (1). The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. However, ICAM-1 is also used as a receptor by the major group of human rhinoviruses and is a catalyst for the subsequent viral uncoating during cell entry (2). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells; its expression is regulated by inflammatory cytokines (1).
Related PathwayES Cell Differentiation/IPSTransmembraneDruggable Genome Cell adhesion molecules (CAMs)Natural killer cell mediated cytotoxicityLeukocyte transendothelial migrationViral myocarditis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ICAM1 antibody [EPR4776]; All lanes : Anti-ICAM1 antibody [EPR4776] at 1/1000 dilution.Lane 1 : Raji cell lysate.Lane 2 : Ramos cell lysate.Lane 3 : A549 cells treated with TNF-alpha, lysate.Lane 4 : A549 cell lysate.Lane 5 : HUVEC cells treated with TNF-alpha, lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 58 kDa.Observed band size : 89 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ICAM1 antibody [EPR4776]; TA307840, at 1/100, staining ICAM1 in Human kidney tissue by immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ICAM1 antibody [EPR4776]; , at 1/100, staining ICAM1 in Human tonsil tissue by immunohistochemistry.
FC Image
Flow Cytometry - Anti-ICAM1 antibody [EPR4776]; Overlay histogram showing Ramos cells stained with TA307840 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title