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Anti-ATG12 Antibody EPR4800
Also for ATG12 (NM_004707)
|A synthetic peptide corresponding to residues on the C-terminus in human Atg12 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||ICC: 1:250 - 1:500; WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens autophagy related 12 (ATG12), transcript variant 1|
|APG12; APG12L; FBR93; HAPG12|
|Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy (1). It is essential for Atg12 conjugation, and the Atg12-Atg5 conjugate is necessary to form autophagic vesicles (2). The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required (3).|
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Western blot - Apg12 antibody [EPR4800]; All lanes : Anti-Apg12 antibody [EPR4800] at 1/1000 dilution.Lane 1 : Human fetal kidney lysate.Lane 2 : HepG2 lysate.Lane 3 : Raji lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 15 kDa.
Western blot - Apg12 antibody [EPR4800]; All lanes : Anti-Apg12 antibody [EPR4800] at 1/1000 dilution.Lane 1 : Apg12 transfected 293T lysate .Lane 2 : Non-transfected 293T lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 15 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apg12 antibody [EPR4800]; ab109491, at 1/250 dilution, staining Apg12 in paraffin-embedded Human breast carcinoma by Immunohistochemistry.