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Anti-IRF9 Antibody EPR3939
Also for IRF9 (NM_006084)
|A synthetic peptide corresponding to residues in human IRF9 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||ICC: 1:100 - 1:250; WB: 1:10000 - 1:50000; FC: 1:10 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens interferon regulatory factor 9 (IRF9)|
|IRF-9; ISGF3; ISGF3G; p48|
|IRF9, or Interferon regulatory factor 9, is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STAT’s then dimerize, associate with IRF9/ISGF3G to form a complex called ISGF3 transcription factor, which enters the nucleus. Moreover, IRF9 is translocated into the nucleus upon activation by IFN-alpha/beta (1).|
Jak-STAT signaling pathway
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Western blot - TF IRF9 antibody [EPR3939]; All lanes : Anti-Interferon regulatory factor 9 antibody [EPR3939] at 1/1000 dilution.Lane 1 : K562 treated with IFN-alpha cell lysates.Lane 2 : K562 cell lysates.Lane 3 : HeLa treated with IFN-alpha cell lysates.Lane 4 : HeLa cell lysates.Lane 5 : Jurkat cell lysates.Lysates/proteins at 10 µg per lane.Predicted band size : 44 kDa.Observed band size : 48 kDa .
Flow Cytometry - TF IRF9 antibody [EPR3939]; Flow cytometric analysis of permeabilized Jurkat cells using ab109309 at 1/10 dilution (red)and Rabbit IgG negative control (green).