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Home Antibody All anti-ACVR1B antibodies

Anti-ACVR1B Antibody EPR4815

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307816
  • Rabbit monoclonal antibody against ACVR1B(clone EPR4815)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401370) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for ACVR1B (NM_004302)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the extracellular domain of human ACVR1B was used as an immunogen.
Clone NameEPR4815 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; FC: 1:100 - 1:500; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 57 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens activin A receptor, type IB (ACVR1B), transcript variant 1
Alternative NameACTRIB; ACVRLK4; ALK4; SKR2
Database LinkNP_004293
Entrez Gene 91 Human
Entrez Gene 11479 Mouse
Entrez Gene 29381 Rat
FunctionACVR1B is an activin A type IB receptor. Activins are dimeric growth and differentiation factors that belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases, which include at least two type I and two type II receptors. ACVR1B is a type I receptor that is essential for signaling. Mutations in this protein are associated with pituitary tumors.
Related PathwayProtein KinaseTransmembraneDruggable Genome MAPK signaling pathwayCytokine-cytokine receptor interactionEndocytosisTGF-beta signaling pathwayAdherens junctionPathways in cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Activin A Receptor Type IB antibody [EPR4815]; All lanes : Anti-Activin A Receptor Type IB antibody [EPR4815] at 1/1000 dilution.Lane 1 : Human brain tissue lysate.Lane 2 : Human fetal kidney.Lane 3 : SH-SY5Y cell lysate.Lane 4 : U87-MG cell lysate.Lane 5 : Human fetal liver.Lysates/proteins at 10 ug per lane.Predicted band size : 57 kDa.Observed band size : 52 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Activin A Receptor Type IB antibody [EPR4815]; TA307816, at 1/500 dilution, staining Activin A receptor type IB in Human kidney tissue by immunohistochemistry.
IF Image
Immunocytochemistry - Anti-Activin A Receptor Type IB antibody [EPR4815]; Alexa Fluor 488 goat anti-rabbit IgG was used as the secondary antibody. The cells (SH-SY5Y) were fixed with 2% paraformaldehyde for 20 min.
FC Image
Flow Cytometry - Anti-Activin A Receptor Type IB antibody [EPR4815]; Overlay histogram showing HEK293 cells stained with TA307816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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