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Home Antibody All anti-RET antibodies

Anti-RET Antibody EPR2871

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307815
  • Rabbit monoclonal antibody against Ret(clone EPR2871)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC412424) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human Ret was used as an immunogen.
Clone NameEPR2871 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:100 - 1:1000; ICC/IF: 1:50 - 1:100
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens ret proto-oncogene (RET), transcript variant 4
Alternative NameCDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1; PTC; RET-ELE1; RET51
Database LinkNP_065681
Entrez Gene 5979 Human
Entrez Gene 19713 Mouse
Entrez Gene 24716 Rat
FunctionRet, a member of the cadherin superfamily, is one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. It plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in Ret are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma.
Related PathwayProtein KinaseTransmembraneDruggable Genome EndocytosisPathways in cancerThyroid cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Ret antibody [EPR2871]; All lanes : Anti-Ret antibody [EPR2871] at 1/10000 dilution.Lane 1 : Neuro-2a cell lysate.Lane 2 : TT cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 124 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871]; Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labelling Ret with TA307815 at 1/250 dilution.
FC Image
Flow Cytometry - Anti-Ret antibody [EPR2871]; Overlay histogram showing Caco 2 cells stained with TA307815 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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