OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-RET antibodies

Anti-RET Antibody EPR2871

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307815 Rabbit monoclonal antibody against Ret(clone EPR2871) 100ul $325 In Stock
LC412424 RET HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

spacer
WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human Ret was used as an immunogen.
Clone NameEPR2871 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:100 - 1:1000; ICC/IF: 1:50 - 1:100
Predicted MW Explanation 124 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens ret proto-oncogene (RET), transcript variant 4
Alternative NameCDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1; PTC; RET-ELE1; RET51
Database LinkNP_065681
Entrez Gene 5979 Human
Entrez Gene 19713 Mouse
Entrez Gene 24716 Rat
FunctionRet, a member of the cadherin superfamily, is one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. It plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in Ret are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma.
Related PathwayProtein KinaseTransmembraneDruggable Genome EndocytosisPathways in cancerThyroid cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-Ret antibody [EPR2871]; All lanes : Anti-Ret antibody [EPR2871] at 1/10000 dilution.Lane 1 : Neuro-2a cell lysate.Lane 2 : TT cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 124 kDa.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871]; Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labelling Ret with TA307815 at 1/250 dilution.
spacer
Flow Cytometry - Anti-Ret antibody [EPR2871]; Overlay histogram showing Caco 2 cells stained with TA307815 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title