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Anti-TSC2 PHOSPHO Antibody EPR1060(2)
Also for TSC2 (NM_000548)
|A phospho specific peptide corresponding to residues surrounding Threonine 1462 of human TSC2/Tuberin was used as an immunogen. This antibody only detects TSC2/Tuberin phosphorylated at Threonine 1462.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
|Does not react with Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens tuberous sclerosis 2 (TSC2), transcript variant 1|
|Tuberous sclerosis complex-2 (TSC2 also known as Tuberin) is a tumor suppressor that forms a complex with TSC1 (Hamartin ) and this complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The C-terminal region of TSC2 contains a GTPase-activating protein (GAP) domain which interacts with Rap1, Rab5 and Rheb (2). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (3). Tuberin is a target for phosphorylation by several kinases including Akt, which directly phosphorylates and inactivates TSC2 on Ser 924, Thr 1462 and Thr 1518. These phosphorylations by Akt disrupt the TSC1-TSC2 complex and disturb the subcellular localization of TSC1 and TSC2. Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 newborns, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene (4).|
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Western blot - Tuberin (phospho T1462) antibody [EPR1060(2)]; All lanes : Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] at 1/1000 dilution.Lane 1 : NIH/3T3 cell lysate.Lane 2 : NIH/3T3 cell lysate treated with insulin.Lysates/proteins at 10 µg per lane.Predicted band size : 201 kDa.Observed band size : 180 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tuberin (phospho T1462) antibody [EPR1060(2)]; Immunohistochemical analysis of paraffin-embedded Human liver tissue using ab109403 at 1/50
Immunocytochemistry/ Immunofluorescence - Tuberin (phospho T1462) antibody [EPR1060(2)]; Immunofluorescent staining of Tuberin in HeLa cells using ab109403 at 1/100.