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Anti-FEN1 Antibody EPR4459(2)
Also for FEN1 (NM_004111)
|A synthetic peptide corresponding to residues in human FEN1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC or IP.
|Homo sapiens flap structure-specific endonuclease 1 (FEN1)|
|FEN-1; MF1; RAD2|
Entrez Gene 2237 Human
Entrez Gene 14156 Mouse
Entrez Gene 84490 Rat
|FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. FEN1 is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by FEN1. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions (1).|
|Stem cell - PluripotencyDruggable Genome DNA replicationBase excision repairNon-homologous end-joining|
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Western blot - Anti-FEN1 antibody [EPR4459(2)]; All lanes : Anti-FEN1 antibody [EPR4459(2)] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 43 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)]; Immunohistochemical analysis of paraffin-embedded Human breast tissue labelled with TA307793 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)]; Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labelled with TA307793 at 1/500 dilution.