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Anti-PPP3CA Antibody EPR1670(2)
Also for PPP3CA (NM_000944)
|A synthetic peptide corresponding to residues near the C-terminus of human PP2B was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||WB: 1:10000 - 1:50000; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IHC-P or IP.
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Western blot - Calcineurin A antibody [EPR1670(2)]; All lanes : Anti-Calcineurin A antibody [EPR1670(2)] at 1/10000 dilution.Lane 1 : fetal brain lysate.Lane 2 : SH-SY5Y lysate.Lane 3 : A431 lysate.Lane 4 : HeLa cells lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 59 kDa.Observed band size : 58 kDa .
Immunocytochemistry/ Immunofluorescence - Calcineurin A antibody [EPR1670(2)]; Immunofluorescent staining of HeLa cells using 1/100 TA307791.
Flow Cytometry - Anti-Calcineurin A antibody [EPR1670(2)]; Overlay histogram showing HeLa cells stained with TA307791 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.