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Anti-PPP3CA Antibody EPR1670(2)


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against PP2B(clone EPR1670(2))
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human PP2B was used as an immunogen.
Clone NameEPR1670(2) IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:10000 - 1:50000; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 59 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P or IP.

Reference Data

Target NameHomo sapiens protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 1
Alternative NameCALN; CALNA; CALNA1; CCN1; CNA1; PPP2B
Database LinkNP_000935
Entrez Gene 5530 Human
Entrez Gene 19055 Mouse
Entrez Gene 24674 Rat
FunctionPP2B, the calcium- and calmodulin-dependent protein phosphatase calcineurin, has been implicated in the transduction of signals that control the hypertrophy of cardiac muscle and slow fiber gene expression in skeletal muscle. Calsarcins represent a novel family of sarcomeric proteins that link calcineurin with the contractile apparatus, thereby potentially coupling muscle activity to calcineurin activation (1). The Z-disc is a highly specialized multiprotein complex of striated muscles that serves as the interface of the sarcomere and the cytoskeleton (2).
Related PathwayPhosphataseDruggable Genome MAPK signaling pathwayCalcium signaling pathwayOocyte meiosisApoptosisWnt signaling pathwayAxon guidanceMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Calcineurin A antibody [EPR1670(2)]; All lanes : Anti-Calcineurin A antibody [EPR1670(2)] at 1/10000 dilution.Lane 1 : fetal brain lysate.Lane 2 : SH-SY5Y lysate.Lane 3 : A431 lysate.Lane 4 : HeLa cells lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 59 kDa.Observed band size : 58 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - Calcineurin A antibody [EPR1670(2)]; Immunofluorescent staining of HeLa cells using 1/100 TA307791.
FC Image
Flow Cytometry - Anti-Calcineurin A antibody [EPR1670(2)]; Overlay histogram showing HeLa cells stained with TA307791 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


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