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Home Antibody All anti-CDK4 antibodies

Anti-CDK4 Antibody EPR4513

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307755
  • Rabbit monoclonal antibody against Cdk4(clone EPR4513)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400021) , 20ug
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for CDK4 (NM_000075)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus in human Cdk4 was used as an immunogen.
Clone NameEPR4513 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000 - 1:10000; ICC/IF: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens cyclin-dependent kinase 4 (CDK4)
Alternative NameCMM3; PSK-J3
Database LinkNP_000066
FunctionCyclin dependent kinases (Cdk's) are kinases that interact with cyclins and regulate cell division. There is evidence that phosphorylation of the C-terminal region of Rb by Cdk4/6 initiates successive intramolecular interactions between the C-terminal region and the central pocket. The initial interaction displaces histone deacetylase from the pocket, blocking active transcriptional repression by Rb. These intramolecular interactions provide a molecular basis for sequential phosphorylation of Rb by Cdk4/6 and Cdk2. Cdk4/6 is activated early in G1, blocking active repression by Rb (1). In primary epidermal cells, it has been found that oncogenic Ras transiently decreases Cdk4 expression in association with cell cycle arrest in G1 phase. Cdk4 co-expression circumvents Ras growth suppression and induces invasive human neoplasia resembling squamous cell carcinoma. Tumorigenesis is dependent on Cdk4 kinase function, with cyclin D1 required but not sufficient for this process. This data identifies a new role for oncogenic Ras in Cdk4 regulation and highlights the functional importance of Cdk4 suppression in preventing uncontrolled growth (2).
Related Pathway
Cell cycle
TGF Beta Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cdk4 antibody [EPR4513]; All lanes : Anti-Cdk4 antibody [EPR4513] at 1/1000 dilution.Lane 1 : Hela cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 34 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Cdk4 antibody [EPR4513]; TA307755, at a 1/100 dilution, staining Cdk4 in HeLa cells by Immunofluorescence.

 

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