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Anti-MSH6 Antibody EPR3945


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SKU Description Amount Price Availability*  
TA307745 Rabbit monoclonal antibody against MSH6(clone EPR3945) 100ul $325 In Stock

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OriGene Data

ImmunogenA synthetic peptide corresponding to residues at the N-terminus in human MSH6 was used as an immunogen.
Clone NameEPR3945 IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens mutS homolog 6 (MSH6), transcript variant 1
Alternative NameGTBP; GTMBP; HNPCC5; HSAP; p160
Database LinkNP_000170
Entrez Gene 2956 Human
Entrez Gene 17688 Mouse
FunctionThe MutS homologue 6 protein (MSH6) is a member of the MutS homolog family required in the DNA mismatch repair system (1). MSH6 forms a MutS alpha dimer with MSH2, binding to DNA mismatches to initiate DNA repair (2). MutS alpha bends the DNA helix and recognizes single base mismatches and dinucleotide insertion-deletion loops in the DNA (2). Heterozygous mutations in the MSH6 gene are a cause of hereditary nonpolyposis colorectal cancer (HNPCC), forming a specific mispair binding complex with MSH3 (3, 4). The frequency of MSH6 mutation is higher in HNPCC than in atypical HNPCC (5). The MSH2/MSH6 dimer may also play a role in DNA homologous recombination repair (2).
Related PathwayStem cell - PluripotencyDruggable Genome Mismatch repairPathways in cancerColorectal cancer

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Western blot - MSH6 antibody [EPR3945]; All lanes : Anti-MSH6 antibody [EPR3945] at 1/1000 dilution.Lane 1 : A431 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : SW480 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 163 kDa.
Other-Anti-MSH6 antibody [EPR3945](TA307745); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MSH6 antibody [EPR3945]; TA307745, at a 1/100 dilution, detecting MSH6 in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemistry. Detection utilised HRP conjugated anti rabbit antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [EPR3945]; TA307745 (1/500) staining MSH6 in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red).
Immunocytochemistry/ Immunofluorescence - MSH6 antibody [EPR3945]; TA307745, at a 1/100 dilution, staining MSH6 in HeLa cells by Immunofluorescence.


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