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Anti-TNNT2 Antibody EPR3696
Also for TNNT2 (NM_000364)
|A synthetic peptide corresponding to residues in human TNNT2 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:20000 - 1:100000; IHC-P: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 1|
|CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TnTC|
Entrez Gene 7139 Human
Entrez Gene 21956 Mouse
Entrez Gene 24837 Rat
|TNNT2 is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. |
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Western blot - Cardiac Troponin T antibody [EPR3696]; All lanes : Anti-Cardiac Troponin T antibody [EPR3696] at 1/20000 dilution.Lane 1 : Human heart lysate.Lane 2 : Human skeletal muscle lysate.Lane 3 : Mouse heart lysate.Lane 4 : Rat heart lysate.Lysates/proteins at 10 ug per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 36 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696]; IHC image ofCardiac Troponin T staining inHuman Heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with TA307718, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.