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Anti-HNF4A Antibody EPR3648
Also for HNF4A (NM_000457)
|A synthetic peptide corresponding to residues in human HNF4 Alpha was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:100; ICC/IF: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2|
|HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14|
|HNF4 (Hepatocyte Nuclear Factor 4) is a nuclear receptor protein belonging to the steroid hormone receptor superfamily. HNF4 is commonly expressed in the liver and plays a critical role in liver development by establishing and maintaining hepatocyte differentiation (1). In human, two isoforms of HNF4 exists, alpha and gamma. HNF4 alpha is transcriptionally controlled transcription factor, which binds to DNA sites required for the transcription of alpha 1- antitrypsin, Apo CIII, transthyretin genes, and HNF1 alpha (2). HNF4 alpha interacts with PGC-1 to activate gluconeogenesis, leading to lipoprotein metabolism regulation (3). HNF4 alpha defects are known to cause type I diabetes (4).|
TGF Beta Signaling Pathway
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Western blot - HNF4 antibody [EPR3648]; All lanes : Anti-HNF4 antibody [EPR3648] at 1/1000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : A549 cell lysate.Lane 3 : SW480 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 53 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HNF4 antibody [EPR3648]; ab92378 staining HNF4 in Formalin-fixed, Paraffin-embedded Human colon tissue (A) and Human kidney tissue (B) using ab92378 at 1/100 dilution. Detection: DAB staining
Immunocytochemistry/ Immunofluorescence - HNF4 antibody [EPR3648]; ab92378 at 1/100 dilution staining HNF4 in HepG2 cells by Immunofluorescence.
Flow Cytometry - Anti-HNF4 antibody [EPR3648]; Overlay histogram showing HepG2 cells stained with ab92378 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.