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Home Antibody All anti-PMS2 antibodies

Anti-PMS2 Antibody EPR3947

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SKU Description Amount Price Availability*  
TA307693
  • Rabbit monoclonal antibody against PMS2(clone EPR3947)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424658) , 20ug Explanation
100ul $325 2 Weeks
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WB(1)
IHC(1)
IF(1)
FC(1)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PMS2 was used as an immunogen.
Clone NameEPR3947 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250; FC: 1:1000
Predicted MW Explanation 96 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens PMS2 postmeiotic segregation increased 2 (S. cerevisiae) (PMS2), transcript variant 1
Alternative NameHNPCC4; PMS2CL; PMSL2
Database LinkNP_000526
Entrez Gene 5395 Human
FunctionMismatch repair endonuclease PMS2 is a member of a family of genes found in clusters on chromosome 7. PMS2 is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in PMS2 are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors (1).
Related PathwayDruggable Genome Mismatch repair

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PMS2 antibody [EPR3947]; All lanes : Anti-PMS2 antibody [EPR3947] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : SH SY5Y cell lysate.Lane 4 : SKBR3 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 96 kDa.Observed band size : 110 kDa .
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Other-Anti-PMS2 antibody [EPR3947](TA307693); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PMS2 antibody [EPR3947]; TA307693 at 1/100 dilution staining PMS2 in Human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
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Immunocytochemistry/ Immunofluorescence - PMS2 antibody [EPR3947]; TA307693 at 1/100 dilution staining PMS2 in HeLa cells by Immunofluorescence.
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Flow Cytometry - Anti-PMS2 antibody [EPR3947]; Overlay histogram showing HeLa cells stained with TA307693 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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