OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-ABAT antibodies

Anti-ABAT Antibody EPR4433

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307681
  • Rabbit monoclonal antibody against ABAT(clone EPR4433)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400218) , 20ug
100ul $325 In Stock
Add to Shopping Cart
WB(1)
IHC(1)
FC(1)
spacer
Also for ABAT (NM_000663)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human ABAT was used as an immunogen.
Clone NameEPR4433 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens 4-aminobutyrate aminotransferase (ABAT), transcript variant 2
Alternative NameGABA-AT; GABAT; NPD009
Database LinkNP_000654
FunctionABAT (4-aminobutyrate aminotransferase) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities (1).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ABAT antibody [EPR4433]; All lanes : Anti-ABAT antibody [EPR4433].Lane 1 : HepG2 cell lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : Human fetal liver tissue lysate.Lane 4 : rat brain tissue lysate.Lane 5 : rat kidney tissue lysate.Lane 6 : mouse brain tissue lysate.Predicted band size : 56 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABAT antibody [EPR4433]; TA307681, at 1/250 dilution, staining ABAT in Human liver tissue by immunohistochemistry.
FC Image
Flow Cytometry - Anti-ABAT antibody [EPR4433]; Overlay histogram showing HepG2 cells stained with TA307681 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title