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Anti-ABAT Antibody EPR4433
Also for ABAT (NM_000663)
|A synthetic peptide corresponding to residues in human ABAT was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens 4-aminobutyrate aminotransferase (ABAT), transcript variant 2|
|GABA-AT; GABAT; NPD009|
|ABAT (4-aminobutyrate aminotransferase) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities (1).|
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Western blot - ABAT antibody [EPR4433]; All lanes : Anti-ABAT antibody [EPR4433].Lane 1 : HepG2 cell lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : Human fetal liver tissue lysate.Lane 4 : rat brain tissue lysate.Lane 5 : rat kidney tissue lysate.Lane 6 : mouse brain tissue lysate.Predicted band size : 56 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABAT antibody [EPR4433]; TA307681, at 1/250 dilution, staining ABAT in Human liver tissue by immunohistochemistry.
Flow Cytometry - Anti-ABAT antibody [EPR4433]; Overlay histogram showing HepG2 cells stained with TA307681 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.