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Home Antibody All anti-RPS6 antibodies

Anti-RPS6 PHOSPHO Antibody EP1338(2)Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307641
  • Rabbit monoclonal antibody against Ribosomal Protein S6 Phospho (Ser235/236) (clone EPTPG3 ) (Phospho-Specific)
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding serine 235/236 of human S6 Ribosomal Protein was used as an immunogen.
Clone NameEP1338(2)Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC: 1:100 - 250, IHC: 1:100 - 250, IP: 1:30, WB: 1:10,000 - 200,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens ribosomal protein S6 (RPS6)
Alternative NameS6
Database LinkNP_001001
Entrez Gene 6194 Human
FunctionRibosomal protein S6 (RPS6) belongs to S6E family of ribosomal proteins and it is involved in the control of cell growth and proliferation via selective translation (1). It is a major substrate of Ribosomal Protein S6 Kinase (RSK) in the eukaryote ribosomes. During translation, it regulates the translation of any RNA which contains 5’ terminal oligopyrimidine sequence (5’TOP) (2). 5’TOP encodes proteins for cell cycle progression, ribosomal proteins, and elongation factors. The phospholyation of RPS6 has been linked to increase in selective 5’TOP translation. The major phospholyation sites RPS6 includes Ser235, 236, 240, and 244 (2). The phospholyation on RPS6 is stimulated by growth factors, tumor promoting agents, and mitogens. During growth arrest, RPS6 is dephoshorylated (3).
Related Pathway RibosomemTOR signaling pathwayInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - RPS6 (phospho S235 + S236) antibody [EP1338(2)Y]; All lanes : Anti-RPS6 (phospho S235 + S236) antibody [EP1338(2)Y] at 1/200000 dilution.Lane 1 : HeLa cell lysate, untreated .Lane 2 : HeLa cell lysate, treated with Calyculin A.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 30 kDa.Observed band size : 30 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPS6 (phospho S235 + S236) antibody [EP1338(2)Y]; TA307641 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [EP1338(2)Y]; ICC/IF image of TA307641 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody TA307641 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

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