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Anti-RPS6 PHOSPHO Antibody EP1338(2)Y
Also for RPS6 (NM_001010)
|A synthetic peptide corresponding to residues surrounding serine 235/236 of human S6 Ribosomal Protein was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC: 1:100 - 250, IHC: 1:100 - 250, IP: 1:30, WB: 1:10,000 - 200,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens ribosomal protein S6 (RPS6)|
|Ribosomal protein S6 (RPS6) belongs to S6E family of ribosomal proteins and it is involved in the control of cell growth and proliferation via selective translation (1). It is a major substrate of Ribosomal Protein S6 Kinase (RSK) in the eukaryote ribosomes. During translation, it regulates the translation of any RNA which contains 5’ terminal oligopyrimidine sequence (5’TOP) (2). 5’TOP encodes proteins for cell cycle progression, ribosomal proteins, and elongation factors. The phospholyation of RPS6 has been linked to increase in selective 5’TOP translation. The major phospholyation sites RPS6 includes Ser235, 236, 240, and 244 (2). The phospholyation on RPS6 is stimulated by growth factors, tumor promoting agents, and mitogens. During growth arrest, RPS6 is dephoshorylated (3). |
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Western blot - RPS6 (phospho S235 + S236) antibody [EP1338(2)Y]; All lanes : Anti-RPS6 (phospho S235 + S236) antibody [EP1338(2)Y] at 1/200000 dilution.Lane 1 : HeLa cell lysate, untreated .Lane 2 : HeLa cell lysate, treated with Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 30 kDa.Observed band size : 30 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPS6 (phospho S235 + S236) antibody [EP1338(2)Y]; ab80158 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [EP1338(2)Y]; ICC/IF image of ab80158 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab80158 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.