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Anti-CD74 Antibody EPR4065
Also for CD74 (NM_001025158)
|A synthetic peptide corresponding to residues in human CD74 was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt.
|Homo sapiens CD74 molecule, major histocompatibility complex, class II invariant chain (CD74), transcript variant 3|
|DHLAG; HLADG; Ia-GAMMA; II|
Entrez Gene 972 Human
|Cluster of Differentiation 74 (CD74), also known as HLA class II histocompatibility antigen gamma chain, is a type II transmembrane protein that plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis (1). It then directs the transport of this complex from the endoplasmic reticulum to compartments where class II peptide loading occurs (1). CD74 serves as a cell surface receptor ligand for the macrophage migration inhibitory factor (MIF) cytokine, which binds to the extracellular domain of CD74 (1, 2). Thus, CD74 is required for MIF-induced activation of the extracellular signal-regulated kinase-1/2 MAP kinase cascade, cell proliferation, and PGE2 production (2). CD74 is also expressed on antigen presenting cells such as B cells (3).|
|TransmembraneDruggable Genome Antigen processing and presentation|
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Western blot - CD74 antibody [EPR4065]; All lanes : Anti-CD74 antibody [EPR4065] at 1/1000 dilution.Lane 1 : Human T-78 cell lysate.Lane 2 : Human Tonsil cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 34 kDa.Observed band size : 31 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD74 antibody [EPR4065]; TA307622, at a dilution of 1/250, staining CD74 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD74 antibody [EPR4065](TA307622); TA307622 showing positive staining in B cell lymphoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD74 antibody [EPR4065](TA307622); TA307622 showing positive staining in Normal thymus tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD74 antibody [EPR4065](TA307622); TA307622 showing negative staining in Normal brain tissue.