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Anti-ERBB4 PHOSPHO Antibody EPR2273(2)
Also for ERBB4 (NM_005235)
|A phospho specific peptide corresponding to residues surrounding Tyrosine 1284 of human HER4/ErbB4 was used as an immunogen. This antibody only detects HER4/ErbB4 phosphorylated at HER4/ErbB4.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4 (ERBB4), transcript variant JM-a/CVT-1|
|ALS19; HER4; p180erbB4|
|HER4/ErbB4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. ErbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). ErbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The HER4/ErbB4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif, known as the PDZ domain (3).|
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Western blot - ErbB 4 (phospho Y1284) antibody [EPR2273(2)]; All lanes : Anti-ErbB 4 (phospho Y1284) [EPR2273(2)] antibody at 1/1000 dilution.Lane 1 : A431 cell lysate, untreated.Lane 2 : A431 cell lysate, treated with Epidermal Growth Factor (EGF) .Lysates/proteins at 10 µg/ml per lane.Predicted band size : 147 kDa.
Immunocytochemistry/ Immunofluorescence - ErbB 4 (phospho Y1284) antibody [EPR2273(2)]; Immunofluorescent staining of A431 cells using ab109273 at a dilution of 1/100.
Flow Cytometry - ErbB 4 (phospho Y1284) antibody [EPR2273(2)]; ab109273 at 1/10 dilution staining ErbB 4 in HeLa cells by Flow cytometry. .Green: Permeabilized A431 cells, untreated .Red: Permeabilized A431 cells, treated with EGF. .Blue: Permeabilized A431 cells, treated with EGF and preincubated with phospho-ErbB 4 (pY1284) peptide .Orange: Permeabilized A431 cells, treated with EGF and preincubated with non-phospho-ErbB 4(pY1284) peptide.