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Anti-PLIN2 Antibody EPR3713
Also for PLIN2 (NM_001122)
|A synthetic peptide corresponding to residues in human ADFP was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:1000 - 1:10000; FC: 1:100 - 1:500; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-Fr or IP.
|Homo sapiens perilipin 2 (PLIN2), transcript variant 1|
|ADFP (Adipose differentiation-related protein) is a major protein associated with the lipid droplets in various types of cells, including macrophage-derived foam cells and lipid-laden liver cells (1). The ADFP protein occurs only transiently in adipocytes. It is present at the surface of nascent lipid droplets in early stages of adipocyte differentiation but reduced dramatically after the onset of perilipin expression, whereas ADFP mRNA remains elevated (2). Up-regulation of ADFP may act to sequester fatty acids as triglycerides in discrete lipid droplets that could protect muscle from the detrimental effects of fatty acids on insulin action and glucose tolerance (3).|
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Western blot - ADFP antibody [EPR3713]; All lanes : Anti-ADFP antibody [EPR3713] at 1/1000 dilution.Lane 1 : Human fetal liver lysate.Lane 2 : HepG2 lysate.Lane 3 : JAR lysate.Lane 4 : Human milk lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 48 kDa.
Western blot - ADFP antibody [EPR3713]; All lanes : Anti-ADFP antibody [EPR3713] at 1/1000 dilution.Lane 1 : Mouse brain lysate.Lane 2 : Rat brain lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 48 kDa.
Immunocytochemistry/ Immunofluorescence - ADFP antibody [EPR3713]; ab108323 at 1/100 dilution staining ADFP in HepG2 cells by Immunofluorescence.
Flow Cytometry - Anti-ADFP antibody [EPR3713]; Overlay histogram showing Jurkat cells stained with ab108323 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.