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Home Antibody All anti-PLIN2 antibodies

Anti-PLIN2 Antibody EPR3713

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307596
  • Rabbit monoclonal antibody against ADFP(clone EPR3713)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400454) , 20ug
100ul 325 In Stock
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WB(2)
IF(1)
FC(1)
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Also for PLIN2 (NM_001122)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human ADFP was used as an immunogen.
Clone NameEPR3713 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; FC: 1:100 - 1:500; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-Fr or IP.

Reference Data

Target NameHomo sapiens perilipin 2 (PLIN2), transcript variant 1
Alternative NameADFP; ADRP
Database LinkNP_001113
FunctionADFP (Adipose differentiation-related protein) is a major protein associated with the lipid droplets in various types of cells, including macrophage-derived foam cells and lipid-laden liver cells (1). The ADFP protein occurs only transiently in adipocytes. It is present at the surface of nascent lipid droplets in early stages of adipocyte differentiation but reduced dramatically after the onset of perilipin expression, whereas ADFP mRNA remains elevated (2). Up-regulation of ADFP may act to sequester fatty acids as triglycerides in discrete lipid droplets that could protect muscle from the detrimental effects of fatty acids on insulin action and glucose tolerance (3).
Related Pathway
Adipogenesis

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ADFP antibody [EPR3713]; All lanes : Anti-ADFP antibody [EPR3713] at 1/1000 dilution.Lane 1 : Human fetal liver lysate.Lane 2 : HepG2 lysate.Lane 3 : JAR lysate.Lane 4 : Human milk lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 48 kDa.
WB Image
Western blot - ADFP antibody [EPR3713]; All lanes : Anti-ADFP antibody [EPR3713] at 1/1000 dilution.Lane 1 : Mouse brain lysate.Lane 2 : Rat brain lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 48 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - ADFP antibody [EPR3713]; TA307596 at 1/100 dilution staining ADFP in HepG2 cells by Immunofluorescence.
FC Image
Flow Cytometry - Anti-ADFP antibody [EPR3713]; Overlay histogram showing Jurkat cells stained with TA307596 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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