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Home Antibody All anti-DNMT1 antibodies

Anti-DNMT1 Antibody EPR3521(2)


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA307586 Rabbit monoclonal antibody against Dnmt1(clone EPR3521(2)) 100ul $325 In Stock
LC427274 DNMT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Dnmt1 was used as an immunogen.
Clone NameEPR3521(2) IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100
BufferpH: 7.40Preservative: 0.01% Sodium azide; Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for ICC/IF or IP.

Reference Data

Target NameHomo sapiens DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1
Alternative NameADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
Database LinkNP_001124295
Entrez Gene 1786 Human
FunctionDnmt1 is one of three families of enzymes (Dnmt1, Dnmt2, Dnmt3 (a and b)) that have a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Abnormal methylation patterns are associated with certain human tumors and developmental abnormalities (1). Dnmt1 is responsible for maintenance of the DNA methylation pattern after DNA replication. Dnmt1 is a large protein and can be subdivided into an N- and a C-terminal part. The C-terminal domain harbors the catalytic center. The N-terminal part of the enzyme comprises several individual domains, and plays a role in intracellular targeting and regulation of the enzyme's activity. Dnmt1 shows a preference for hemimethylated CG sites and the specificity of Dnmt1 for hemimethylated target sites is a key property for that function (2).
Related PathwayTranscription FactorsDruggable Genome Cysteine and methionine metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Dnmt1 antibody [EPR3521(2)]; All lanes : Anti-Dnmt1 antibody [EPR3521(2)] at 1/1000 dilution.Lane 1 : Jurkat cell lysates.Lane 2 : 293T cell lysates.Lane 3 : HeLa cell lysates.Lane 4 : HuT-78 cell lysates.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 183 kDa.

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)]; Immunohistochemical analysis of paraffin embedded Human stomach tissue labelled with TA307586 at 1/250 dilution.

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [EPR3521(2)]; Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with TA307586 at 1/250 dilution.


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