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Home Antibody All anti-KI67 antibodies

Anti-KI67 Antibody EPR3610

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307570
  • Rabbit monoclonal antibody against Ki-67 (clone EPR3610 )
100ul 325 3-7 Days
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WB(1)
IHC(6)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Ki-67 was used as an immunogen.
Clone NameEPR3610 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:500 - 1:1000; IHC-P: 1:500 - 1:1000; FC: 1:100 - 1:550; ICC/IF: 1:250 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens marker of proliferation Ki-67 (MKI67), transcript variant 2
Alternative NameKIA; MIB-; MIB-1; PPP1R105
Database LinkNP_001139438
Entrez Gene 4288 Human
FunctionKi-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized in the perinucleolar region and also found in nuclear matrix in the later phases (1). Ki-67 is thought to be involved in the maintaining of cell proliferation, however, its exact mechanism for function is unknown. Due to the fact that Ki-67 can be used to determine the growth fraction (the proportion of the cells committed to the cell cycle), it is consider an important marker in human tumor diagnostics and prognostics (2).
Related PathwayES Cell Differentiation/IPSDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Ki67 antibody [EPR3610]; Anti-Ki67 antibody [EPR3610] at 1/500 dilution + HeLa lysates at 10 ug.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 359 kDa.Observed band size : 395 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ki67 antibody [EPR3610]; TA307570, at a 1/500 dilution, staining Ki67 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ki67 antibody [EPR3610]; TA307570, at a 1/500 dilution, staining Ki67 in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ki67 antibody [EPR3610](TA307570); TA307570 showing positive staining in Colonic adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ki67 antibody [EPR3610](TA307570); TA307570 showing positive staining in Normal colon tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ki67 antibody [EPR3610](TA307570); TA307570 showing positive staining in Ovarian carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ki67 antibody [EPR3610](TA307570); TA307570 showing positive staining in Cervical carcinoma tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Ki67 antibody [EPR3610]; TA307570, at a 1/250 dilution, staining Ki67 in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry-Anti-Ki67 antibody [EPR3610](TA307570); Overlay histogram showing Ramos cells stained with TA307570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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