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Home Antibody All anti-CHEK1 antibodies

Anti-CHEK1 Antibody EPR3952

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307552
  • Rabbit monoclonal antibody against Chk1(clone EPR3952)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400510) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Chk1 was used as an immunogen.
Clone NameEPR3952 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Purification pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3
Alternative NameCHK1
Database LinkNP_001265
Entrez Gene 1111 Human
FunctionChk1 is a serine/threonine-protein kinase required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA (1). It is regulated by Atr and plays an important role in embryonic development and tumor suppression (2). The activated Chk1 triggers the G2/M checkpoint, which arrests the cells in G2 to allow them to repair their DNA (3). The inhibition of Chk1 kinase results in the abrogation of the G2/M checkpoint and leads to cell death (3). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (4). Activated Chk1 can bind to, phosphorylate (at serine 216), and inactivate cdc25c (4, 5).
Related PathwayProtein KinaseStem cell - PluripotencyDruggable Genome Cell cyclep53 signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Chk1 antibody [EPR3952]; All lanes : Anti-Chk1 antibody [EPR3952] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : K562 cell lysate.Lane 3 : MCF-7 cell lysate.Lane 4 : HepG52 cell lysate.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.

 

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