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Anti-CHEK1 Antibody EPR3952
Also for CHEK1 (NM_001274)
|A synthetic peptide corresponding to residues in human Chk1 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC,IHC-P or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3|
|Chk1 is a serine/threonine-protein kinase required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA (1). It is regulated by Atr and plays an important role in embryonic development and tumor suppression (2). The activated Chk1 triggers the G2/M checkpoint, which arrests the cells in G2 to allow them to repair their DNA (3). The inhibition of Chk1 kinase results in the abrogation of the G2/M checkpoint and leads to cell death (3). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (4). Activated Chk1 can bind to, phosphorylate (at serine 216), and inactivate cdc25c (4, 5).|
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Western blot - Anti-Chk1 antibody [EPR3952]; All lanes : Anti-Chk1 antibody [EPR3952] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : K562 cell lysate.Lane 3 : MCF-7 cell lysate.Lane 4 : HepG52 cell lysate.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.